The PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, αIIbβ3) complex of activated platelets at or near the platelet fibrinogen receptor.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Flow Cytometry
Flow-cytometry using anti-human PAC-1 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PAC-1 antibody monoclonal antibody (Catalog # TD-HY355407 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human PAC-1 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PAC-1 antibody monoclonal antibody (Catalog # TD-HY355407 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.