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Anti-Human CD172a/SIRPA Antibody (18D5)
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| Catalog No. | TD-HX892107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Clone ID | 18D5 |
| Clonality | Monoclonal |
| Target | SIRPα, BIT, Signal-regulatory protein alpha-1, Brain Ig-like molecule with tyrosine-based activation motifs, MFR, SIRP, Sirp-alpha-1, Sirp-alpha-2, PTPNS1, SIRPA, SHP substrate 1, CD172 antigen-like family member A, Sirp-alpha-3, Inhibitory receptor SHPS-1, Signal-regulatory protein alpha-3, CD172a, Tyrosine-protein phosphatase non-receptor type substrate 1, MyD-1 antigen, Bit, SHPS-1, Macrophage fusion receptor, Signal-regulatory protein alpha-2, SHPS1, MYD1, p84 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P78324 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Detects CD172a/SIRPA in indirect ELISAs. |
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Flow-cytometry using anti-human CD172a antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a antibody monoclonal antibody (Catalog # TD-HX892107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD172a antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a antibody monoclonal antibody (Catalog # TD-HX892107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD172a antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a monoclonal antibody (Catalog: TD-HX892107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC ((Catalog: TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |