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Anti-Human DSG3 Antibody (DF151)
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| Catalog No. | TD-HW575107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Clone ID | DF151 |
| Clonality | Monoclonal |
| Target | Cadherin family member 6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, CDHF6 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P32926 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Detects DSG3 in indirect ELISAs. |
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Flow-cytometry using anti-human DSG3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DSG3 antibody monoclonal antibody (Catalog # TD-HW575107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Western blot analysis was performed using anti-human DSG3 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human DSG3 (Catalog No: TD-HW575011) Lane 2: negative control |