TD-HW575107

Anti-Human DSG3 Antibody (DF151)(TD-HW575107) is amouse monoclonalantibody detectingCadherin family member 6inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Mouse

IgG1, kappa

DF151

Monoclonal

Cadherin family member 6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, CDHF6

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P32926

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects DSG3 in indirect ELISAs.　　|　Flow-cytometry using anti-human DSG3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DSG3 antibody monoclonal antibody (Catalog No.:TD-HW575107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Western blot analysis was performed using anti-human DSG3 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human DSG3 (Catalog No.:TD-HW575011)Lane 2:negative control

For research use only.