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Anti-Human CD146/MUC18/MCAM Antibody (C3.19.1)
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| Catalog No. | TD-HW484107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | C3.19.1 |
| Clonality | Monoclonal |
| Target | CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P43121 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Flow-cytometry using anti-human CD146 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # TD-HW484107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human CD146 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # TD-HW484107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Western blot analysis was performed using anti-CD146/MCAM monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD146/MCAM (Catalog No: TD-HW484011) Lane 2: negative control |