Protein A/G purified from cell culture supernatant.
Accession
P48061
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Bioactivity
Detects Recombinant Human CXCL12/SDF-1 Protein (Cat No.: TD-HW376012) in indirect ELISA.
Flow Cytometry
Flow-cytometry using anti-human CXCL12 antibody.MDA-MB-453 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CXCL12 antibody monoclonal antibody (Catalog # TD-HW376107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.