TD-HW310053

Human

ELISA, FCM, WB

Rat

IgG1, kappa

YP7

Monoclonal

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P51654

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　WB

1:2000-1:20000　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects recombinant human Glypican-3/GPC3 (Catalog No.:TD-HW310011) in indirect ELISA.,Detects recombinant mouse Glypican-3/GPC3 (Catalog No.:TD-MW310011) in indirect ELISA.　　|　　SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).　　|　　Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog No.:TD-HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-HW310053) and cells analysed on a NovoCyte Flow Cytometer.　　|　　Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human GPC3 (Catalog No.:TD-HW310011)Lane 2:negative control

For research use only.