TD-HW310023

Human, Mouse

ELISA, FCM, WB

Human

IgG1, kappa

YP7

Monoclonal

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P51654

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　WB

1:2000-1:20000　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Detects Human GPC3 in indirect ELISAs.　　|　　Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog No.:TD-HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog No.:TD-HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog No.:TD-HW310023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human GPC3 Antibody (YP7).　　|　　Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog No.:TD-HW310023) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1µg/ml on various samples.Lane 1:recombinant mouse GPC3/Glypican 3 (Catalog No.:TD-MW310011)Lane 2:negative control,Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.Lane 1:HepG2 cell lysate

For research use only.