Anti-CTNNB1 (S33F) Mouse Monoclonal Antibody

Catalog  Number: TD-26304
Gene  Symbol:  Beta-catenin,  CTNNB,  Catenin  beta-1 
Description:  Anti-CTNNB1  (S33F)  Mouse  Monoclonal Antibody
Background:  CTNNB1  protein  is  a dual function protein.  It is  a subunit  of  a complex  of  proteins that from adherens  junctions,  which  are important for  the  establishment  and  maintenance  of epithelial  cell  layers by  regulating  cell  growth and  adhesion  between  adjacent  cells. CTNNB1 protein  also pulls double  duty  as an  intracellular signal transducer  in  the Wnt signaling  pathway. Mutations of  CTNNB1  have  been  implicated  in the pathogenesis  of  several cancers.
Immunogen: A synthetic  peptide  from  the internal region  of  CTNNB1  which  includes the mutation  of  S33F,  human  origin.
Tested applications: ELISA,  WB,  IHC 
Recommended dilutions: ELISA:  1:1000-1:5000、WB:  1:500-1:1000、IHC: 1:50-1:100 
Concentration:  2  mg/ml 
Host: Mouse 
Clonality:  Monoclonal 
Purity: Purified  from ascites 
Format: Liquid
Storage buffer: 
Preservative: no
Constituents:  PBS  (without  Mg2+   and  Ca2+),  pH 7.4, 150  mM  NaCl,  50% glycerol
Species Reactivity: Recognizes  S33F mutant, but  not  wild-type  CTNNB1  of  vertebrates. 
Storage Conditions:  Store  at -20°C. Avoid freeze  / thaw  cycles.

Western blot:	Western blot  analysis of  recombinant CTNNB1 (S33F)  and  wildtype proteins. Purified  His-tagged  CTNNB1  (S33F) protein (lane2)  and  corresponding  wild-type  protein (lane1)  were  blotted  with  anti-CTNNB1  (S33F) monoclonal antibody  (Cat. #TD-26304).
Immunofluorescence:	Immunofluorescence of cells expressing CTNNB1 proteins with anti-CTNNB1 (S33F) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (S33F) plasmid (right column), then fixed and stained with anti-CTNNB1 (S33F) monoclonal antibody (Cat. #TD-26304)