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CD322
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- Catalog： TD-31590
- Clonality： mAb
- Application： WB, IF, FCM
- Synonyms： JAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
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规格：
100ul 50ul
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价格：
¥2180
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Mouse Monoclonal Antibody to CD322
| Description | |
| This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. |
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| Specification | |
| Aliases | JAM2; JAMB; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43 |
| Entrez GeneID | 58494 |
| Swissprot | P57087 |
| clone | 7E4C5 |
| WB Predicted band size | 33.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD322 (AA: extra 29-238) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
| WB | 1/500 - 1/2000 |
| IF | 1/100 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
| Product Image | |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using CD322 mouse mAb against NIH/3T3 (1), Ramos (2), and HepG2 (3) cell lysate.
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Immunofluorescence analysis of Hela cells using CD322 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of HL-60 cells using CD322 mouse mAb (green) and negative control (red).
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