Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Store at 4°C for 12 months. Protect from light. Do not freeze.
Note
For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Flow CytoMetry
Flow-cytometry using FITC anti-human VIM antibody. U251 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VIM monoclonal antibody (Catalog: TD-HY550117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.