Anti-Human VIM/Vimentin Antibody (VB6)(TD-HY550107) is ahuman monoclonalantibody detectingVimentininELISA, FCM, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications.
Species reactivity
Human
Applications
ELISA, FCM, WB
Host species
Human
Isotype
IgG1, kappa
Clone ID
VB6
Clonality
Monoclonal
Target
Vimentin, VIM, RHC35301
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P08670
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
SEC-HPLC detection for Anti-Human VIM/Vimentin Antibody (VB6). | Flow-cytometry using anti-human VIM antibody.VIM/Vimentin Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog No.:TD-HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. | Flow-cytometry using anti-human VIM antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog No.:TD-HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.
抗体
试剂盒
多肽
蛋白
组织芯片
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