TD-HY422107

Anti-Human CD28 Antibody (SAA0010)(TD-HY422107) is ahuman monoclonalantibody detectingCD28inELISA, FCM, IHC, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM, IHC, WB

Human

IgG4

SAA0010

Monoclonal

CD28, TP44, T-cell-specific surface glycoprotein CD28, RHC83401

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P10747

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SEC-HPLC detection for Anti-Human CD28 Antibody (ABS1952).　　|　Flow-cytometry using anti-human CD28 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD28 antibody monoclonal antibody (Catalog No.:TD-HY422107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD28 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD28 monoclonal antibody (Catalog No.:TD-HY422107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.