TD-HY286307

Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21)(TD-HY286307) is amouse monoclonalantibody detectingp185erbB2inELISA, FCM, IHC, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM, IHC, WB

Mouse

IgG2a, kappa

A21

Monoclonal

p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P04626

Liquid

0.01M PBS, pH 7.4, 0.09% Sodium azide.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human ERBB2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog No.:TD-HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog No.:TD-HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog No.:TD-HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human ERBB2 antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog No.:TD-HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.