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流式同型对照抗体
Anti-Human OPN/SPP1 Antibody (SAA0512)
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  • CatalogTD-HY207107
  • ClonalitymAb
  • Application ELISA, FCM, SPR, WB
  • Synonyms
  • 规格
    50ug
  • 价格
    ¥询价
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Catalog No. TD-HY207107
Species reactivity Human
Applications ELISA, FCM, SPR, WB
Host species Mouse
Isotype IgG2a, kappa
Clone ID SAA0512
Clonality Monoclonal
Target Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P10451
Form Liquid
Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.

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SDS-PAGE
 
SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512)
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Flow Cytometry
 
Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # TD-HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Western blot
 
Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OPN/SPP1 (Catalog No: TD-HY207012)
Lane 2: negative control