TD-HY207107

Anti-Human OPN/SPP1 Antibody (SAA0512)(TD-HY207107) is amouse monoclonalantibody detectingOsteopontininELISA, FCM, SPR, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM, SPR, WB

Mouse

IgG2a, kappa

SAA0512

Monoclonal

Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P10451

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512)　　|　Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog No.:TD-HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human OPN/SPP1 (Catalog No.:TD-HY207012)Lane 2:negative control

For research use only.