TD-HY196207

Anti-Human MET/c-Met/HGFR Antibody (224G11)(TD-HY196207) is ahuman monoclonalantibody detectingTyrosine-protein kinase MetinFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Human

IgG1, kappa

224G11

Monoclonal

Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P08581

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human c-Met antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog No.:TD-HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human c-Met antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog No.:TD-HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human c-Met antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog No.:TD-HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.