Anti-Human CD220/INSR Antibody (AGT-181)(TD-HY112107) is amouse monoclonalantibody detectingINSRinFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.
Species reactivity
Human
Applications
FCM
Host species
Mouse
Isotype
IgG2a, kappa
Clone ID
AGT-181
Clonality
Monoclonal
Target
INSR, IR, CD220, Insulin receptor
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P06213
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
Flow-cytometry using anti-human CD220 antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD220 antibody monoclonal antibody (Catalog No.:TD-HY112107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. | Flow-cytometry using anti-human CD220 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD220 antibody monoclonal antibody (Catalog No.:TD-HY112107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.