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Anti-Human CD3 Antibody (UCHT1)
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| Catalog No. | TD-HY057607 |
|---|---|
| Description | 7 |
| Species reactivity | Human |
| Applications | FCM |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | UCHT1 |
| Clonality | Monoclonal |
| Target | T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P07766 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Flow-cytometry using anti-human CD3E antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # TD-HY057607 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. |