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流式同型对照抗体
Anti-Human CD3E Antibody (YTH12.5)
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  • CatalogTD-HY057107
  • ClonalitymAb
  • Application FCM
  • Synonyms
  • 规格
    50ug
  • 价格
    ¥询价
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Catalog No. TD-HY057107
Species reactivity Human
Applications FCM
Host species Mouse
Isotype IgG1, kappa
Clone ID YTH12.5
Clonality Monoclonal
Target T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P07766
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.

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Bioactivity
 
SEC-HPLC detection for Anti-Human CD3E Antibody (YTH12.5).
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Flow Cytometry
 
Flow-cytometry using anti-human CD3E antibody.CD3E Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # TD-HY057107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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SDS-PAGE
 
SDS-PAGE for Anti-Human CD3E Antibody (YTH12.5).