Anti-Human CD81 Antibody (5A6)(TD-HX855107) is amouse monoclonalantibody detectingCD81 antigeninFCM, WB. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.
Species reactivity
Human
Applications
FCM, WB
Host species
Mouse
Isotype
IgG1, kappa
Clone ID
5A6
Clonality
Monoclonal
Target
CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P60033
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
Detects CD81 in indirect ELISAs. | Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog No.:TD-HX855107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. | Western blot analysis was performed using anti-CD81 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD81 (Catalog No.:TD-HX855011)Lane 2:negative control