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流式同型对照抗体
Anti-Human CD81 Antibody (5A6)
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  • CatalogTD-HX855107
  • ClonalitymAb
  • Application FCM, WB
  • Synonyms
  • 规格
    50ug
  • 价格
    ¥询价
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Catalog No. TD-HX855107
Species reactivity Human
Applications FCM, WB
Host species Mouse
Isotype IgG1, kappa
Clone ID 5A6
Clonality Monoclonal
Target CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P60033
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.

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Bioactivity
 
Detects CD81 in indirect ELISAs.
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Flow Cytometry
 
Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog # TD-HX855107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Western blot
 
Western blot analysis was performed using anti-CD81 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD81 (Catalog No: TD-HX855011)
Lane 2: negative control