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流式同型对照抗体
Anti-Human NECTIN4/PVRL4 Antibody (SAA0403)
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  • CatalogTD-HV985207
  • ClonalitymAb
  • Application ELISA, FCM
  • Synonyms
  • 规格
    50ug
  • 价格
    ¥询价
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Catalog No. TD-HV985207
Species reactivity Human
Applications ELISA, FCM
Host species Mouse
Isotype IgG2a, kappa
Clone ID SAA0403
Clonality Monoclonal
Target LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q96NY8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.

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Bioactivity
 
Detects NECTIN4/PVRL4 in indirect ELISAs.
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Flow CytoMetry
 
Flow-cytometry using anti-human NECTIN4 antibody.NECTIN4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NECTIN4 antibody monoclonal antibody (Catalog # TD-HV985207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Flow CytoMetry
 
Flow-cytometry using anti-human NECTIN4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human NECTIN4 monoclonal antibody (Catalog: TD-HV985207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog: TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Western blot
 

Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NECTIN4 (Catalog No: TD-HV985011)
Lane 2: negative control