TD-HT182107

Anti-Human CD156c/ADAM10 Antibody (11G2)(TD-HT182107) is amouse monoclonalantibody detectingADAM 10inFCM, WB. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM, WB

Mouse

IgG1, kappa

11G2

Monoclonal

ADAM 10, Mammalian disintegrin-metalloprotease, CD156c, MADM, ADAM10, Kuzbanian protein homolog, KUZ, CDw156, Disintegrin and metalloproteinase domain-containing protein 10

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

O14672

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human CD156c antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog No.:TD-HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD156c antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog No.:TD-HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog No.:TD-HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog No.:TD-HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Western blot analysis was performed using anti-CD156c/ADAM10 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human CD156c/ADAM10 (Catalog No.:TD-HT182012), 50ngLane 2:recombinant human CD156c/ADAM10 (Catalog No.:TD-HT182012), 30ng

For research use only.