Anti-Human CD277/BTN3A1 Antibody (103.2#)(TD-HT139107) is amouse monoclonalantibody detectingBTF5inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.
Species reactivity
Human
Applications
FCM
Host species
Mouse
Isotype
IgG1, kappa
Clone ID
103.2#
Clonality
Monoclonal
Target
BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O00481
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternate Names
hu103.2
Caption
Detects CD277/BTN3A1 in indirect ELISAs. | Flow-cytometry using anti-human CD277 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD277 antibody monoclonal antibody (Catalog No.:TD-HT139107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. | SDS-PAGE for Anti-Human CD277/BTN3A1 Antibody (103.2#) | Western blot analysis was performed using anti-CD277/BTN3A1 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human CD277/BTN3A1 (Catalog No.:TD-HT139011)Lane 2:negative control