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AKT1 mouse monoclonal antibody
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TD-AB0022
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AKT1 mouse monoclonal antibody
| Product Name | AKT1 mouse monoclonal antibody |
| Cat.# | TD-AB0022 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti-AKT1 mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of human AKT1 |
| Reactivity | Human |
| Host species | Mouse |
| Ig class | IgG1 |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | AKT1 |
| Full name | V-akt murine thymoma viral oncogene homolog 1 |
| Target Background | |
| The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. | |
| Western blotting | |
| Predicted band size | 56 kDa |
| Positive control | Hela, 231, PC-3 and HepG2 cell lysates |
| Recommended dilution | 1: 1000-5000 |
| Gel: | 8%SDS-PAGE |
| Lysate: | 40 μg |
| Lane 1-4: | Hela, 231, PC-3 and HepG2 cell lysates |
| Primary antibody: | D191053(AKT1 Antibody) at dilution 1/1200 |
| Secondary antibody: | Goat anti mouse IgG at 1/5000 dilution |
| Exposure time: | 2 minutes |
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