Product Name |
PRKAA1 mouse monoclonal antibody |
Cat.# |
TD-AB0095 |
Package |
25 μl/100 μl/200 μl |
Storage |
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
Product Imformation |
Description |
Anti- PRKAA1 mouse monoclonal antibody |
Applications |
WB |
Immunogen |
Fusion protein of human PRKAA1 |
Reactivity |
Human, Mouse, Rat, Monkey |
Host species |
Mouse |
Ig class |
IgG1 |
Purity |
Protein A/G Affinity Purification |
Target information |
Symbol |
PRKAA1 |
Full name |
protein kinase AMP-activated catalytic subunit alpha 1 |
Synonyms |
AMPK; AMPKa1 |
Target Background |
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-primeAMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Western blotting |
Predicted band size |
64 kDa |
Positive control |
Jurkat, Hela, HepG2, MCF-7, COS-7, NIH/3T3, K562, HEK293 and PC-12 cell lysates |
Recommended dilution |
500-2000 |
Gel |
8%SDS-PAGE |
Lysate |
20 μg |
Lane 1-9 |
Jurkat, Hela, HepG2, MCF-7, COS-7,NIH/3T3, K562, HEK293 and PC-12 cell lysates |
Primary antibody |
D194951(PRKAA1 Antibody) at dilution 1/1000 |
Secondary antibody |
HRP-conjugated Goat anti mouse IgG at 1/10000 dilution |
Exposure time |
2 minutes |
Product Image |
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