TD-HF802107

Anti-Human IL1A/IL1F1 Antibody (SAA0366)(TD-HF802107) is amouse monoclonalantibody detectingIL1F1inELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

ELISA, FCM

Mouse

IgG1, kappa

SAA0366

Monoclonal

IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P01583

Liquid

0.01M PBS, pH 7.4, 0.09% Sodium azide.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human IL1A antibody.IL1A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog No.:TD-HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human IL1A antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog No.:TD-HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human IL1A antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog No.:TD-HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog No.:TD-HF802107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.