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Anti-Human IL33 Antibody (SAA0395)
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| Catalog No. | TD-HF543107 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM |
| Host species | Mouse |
| Isotype | IgG1, lambda |
| Clone ID | SAA0395 |
| Clonality | Monoclonal |
| Target | NF-HEV, IL-1F11, C9orf26, IL-33, Interleukin-1 family member 11, IL33, Nuclear factor from high endothelial venules, NFHEV, Interleukin-33, IL1F11 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | O95760 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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Flow-cytometry using anti-human IL33 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # TD-HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human IL33 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # TDHF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
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Flow-cytometry using anti-human IL33 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL33 antibody monoclonal antibody (Catalog # TD-HF543107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. |