TD-HF004207

Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3)(TD-HF004207) is ahuman monoclonalantibody detectingHER1inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Human

IgG2, kappa

E7.6.3

Monoclonal

HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P00533

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human EGFR antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog No.:TD-HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human EGFR antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog No.:TD-HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human EGFR antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog No.:TD-HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.