TD-HC513107

Anti-Human NOTCH2/3 Antibody (SAA0143)(TD-HC513107) is ahuman monoclonalantibody inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Human

IgG2, kappa

SAA0143

Monoclonal

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q04721, Q9UM47

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human NOTCH2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog No.:TD-HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human NOTCH3 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH3 antibody monoclonal antibody (Catalog No.:TD-HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human NOTCH2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog No.:TD-HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human NOTCH2 & NOTCH3 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 & NOTCH3 antibody monoclonal antibody (Catalog No.:TD-HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.