TD-HC359207

Anti-Human CD47/MER6 Antibody (B6H12)(TD-HC359207) is amouse monoclonalantibody detectingLeukocyte surface antigen CD47inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Mouse

IgG1, kappa

B6H12

Monoclonal

Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q08722

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SEC-HPLC detection for Anti-Human CD47/MER6 Antibody (B6H12).　　|　Detects CD47/MER6 in indirect ELISAs.　　|　Flow-cytometry using anti-human CD47 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog No.:TD-HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD47 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog No.:TD-HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD47 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog No.:TD-HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD47 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog No.:TD-HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human CD47 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD47 monoclonal antibody (Catalog No.:TD-HC359207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.