TD-HB684107

Anti-Human CD104/ITGB4 Antibody (65A12)(TD-HB684107) is amouse monoclonalantibody detectingGP150inFCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.

Human

FCM

Mouse

IgG1, kappa

65A12

Monoclonal

GP150, Integrin beta-4, CD104, ITGB4

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P16144

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Flow-cytometry using anti-human ITGB4 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog No.:TD-HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human ITGB4 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog No.:TD-HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　Flow-cytometry using anti-human ITGB4 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog No.:TD-HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only.