TD-HY328043

Human

ELISA, FCM, WB

Human

IgG4, kappa

SAA2009

Monoclonal

Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P05231

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ELISA　|　WB

1:2000-1:20000　|　1:500-1:2000

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

SEC-HPLC detection for Anti-Human IL6 Antibody (ABS1221).　　|　　Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog No.:TD-HY328043 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.　　|　　SDS-PAGE for Anti-Human IL6 Antibody (SAA2009).　　|　　Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human IL6 (Catalog No.:TD-HY328021), 50ngLane 2:recombinant human IL6 (Catalog No.:TD-HY328021), 30ngLane 3:recombinant human IL6 (Catalog No.:TD-HY328021), 20ngLane 4:recombinant human IL6 (Catalog No.:TD-HY328021), 10ng

For research use only.