Target
Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor
Caption
SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244). | Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog No.:TD-HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer.,Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog No.:TD-HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog No.:TD-HF690414) and cells analysed on a NovoCyte Flow Cytometer. | SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022). | Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.Lane 1:recombinant human IL6 (Catalog No.:TD-HY328021), 50ngLane 2:recombinant human IL6 (Catalog No.:TD-HY328021), 30ngLane 3:recombinant human IL6 (Catalog No.:TD-HY328021), 20ngLane 4:recombinant human IL6 (Catalog No.:TD-HY328021), 10ng