Protein A/G purified from cell culture supernatant.
Accession
P02751
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA | WB
Dilution
1:2000-1:20000 | 1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522) | Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog No.:TD-HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (Catalog No.:TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer.