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Anti-Ras(G12D) Mouse Monoclonal Antibody
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- Catalog： TD-26036
- Clonality： mAb
- Application： ELISA, WB, IF, IHC
- Synonyms： Ras(G12D)
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规格：
100ul
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价格：
¥4800
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Anti-Ras(G12D) Mouse Monoclonal Antibody
| Cat. #: TD-26036 | |
| Gene Symbol: RAS(G12D) | |
| Description: Anti-Ras(G12D) Mouse Monoclonal Antibody | |
| Background: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. | |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin | |
| Applications: ELISA, WB, IF, IHC | |
| Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG1, IgG2a, IgG2b | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
| Western blot:
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| Immunofluorescence:
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Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(WT) plasmid (left column) or pCDNA3-GFP Ras(G12D) plasmid (right column), then fixed and stained with anti-Ras(G12D) monoclonal antibody (Cat. # TD-26036). |