Cat. #: TD-26038 |
Gene Symbol: Ras(G13D) |
Description: Anti-Ras(G13D) Mouse Monoclonal Antibody |
Background: Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin. |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG2b |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% glycerol |
Species Reactivity: Anti-Ras(G13D) monoclonal antibody recognizes RasG13D proteins of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Immunofluorescence:
Immunofluorescence of cells expressing RAS proteins with Anti-Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # TD-26038). |