Catalog  Number: TD-26039
Gene  Symbol:  B - Raf   (V600E)
Description: Anti- B - Raf (V600E) Mouse Monoclonal Antibody

Background:    B-Raf is a member of  the Raf family of  Ser/Thr protein kinases.  It functions downstream of Ras to regulate the MAP kinase signaling pathway.  Mutations in the BRAF gene cause  diseases.   Inherited     mutations in BRAF cause  cardiofaciocutaneous syndrome . Acquired mutations in BRAF have been found in cancers 
Immunogen:    A synthetic  peptide from the internal region  of  BRaf(V600E)， human  origin. 
Tested applications:        ELISA,  WB,  IHC ,  IP 
Recommended dilutions:
ELISA 1:100-1:3000 
WB 1:200-1:1000 
IHC 1:10-1:1000 
Concentration: 0.5 mg/ml 
Host: mouse
Clonality:  Monoclonal 
Isotype: IgG2b
Purity: Purified  from ascites 
Format: Liquid
Storage buffer: 
Preservative: no
Constituents:  PBS  (without  Mg2+   and  Ca2+),  pH 7.4, 150  mM  NaCl,  50%  glycerol
Species Reactivity:     Recognizes  B-Raf(V600E), but  not  wild  type  B-Raf proteins  from vertebrates.
Storage Conditions:  Store  at -20°C. Avoid freeze  / thaw  cycles

Western blot:

Immunofluorescence: