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Anti-ErbB2 (V777A) mouse monoclonal Antibody
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TD-26080
- Catalog： TD-26080
- Clonality： mAb
- Application： ELISA, WB, IF, IHC
- Synonyms： ErbB2(V777A)
-
规格：
100ul
-
价格：
¥4800
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Anti-ErbB2 (V777A) mouse monoclonal Antibody
| Cat. #: TD-26080 | |
| Gene Symbol: ERBB2/HER2/Neu | |
| Description: Anti-ErbB2(V777A) Mouse Monoclonal Antibody | |
| Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers. | |
| Immunogen: A synthetic peptide surround the 777A codon of ErbB2 protein. | |
| Tested Applications: ELISA, WB, IF, IHC | |
| Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IF: 1:100 IHC: 1:50-1:100 | |
| Concentration: 0.2 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. | |
| Immunofluorescence:
|
Immunofluorescence of cells expressing ErbB2 proteins with Anti-ErbB2(V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2(V777A) plasmid (right column), then fixed and stained with Anti-ErbB2(V777A) monoclonal antibody (Cat. #TD-26080). |