抗体
试剂盒
多肽
探针
蛋白
组织芯片
产品中心
Product Center
Anti-IDH1 (R132H) Mouse Monoclonal Antibody
All
TD-260811
TD-260812
- Catalog： TD-26081
- Clonality： mAb
- Application： ELISA, WB, IHC, IF
- Synonyms： IDH1(R132H)
-
规格：
100ul
-
价格：
¥4800
定制服务咨询
Anti-IDH1 (R132H) Mouse Monoclonal Antibody
| Cat. #: TD-26081 | |
| Gene Symbol: IDH1 | |
| Description: Anti-IDH1(R132H) Mouse Monoclonal Antibody | |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. | |
| Immunogen: Recombinant protein of human IDH1 which includes the mutation of R132H. | |
| Applications: ELISA, WB, IHC, IF | |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
|
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
|
Western blot:
Immunofluorescence:
|
Western blot analysis of recombinant IDH1(R132H) and wild type proteins.
Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1(R132H) plasmid (right column), then fixed and stained with Anti-IDH1(R132H) monoclonal antibody (Cat. # TD-26081). |