抗体
试剂盒
多肽
探针
蛋白
组织芯片
- Catalog： TD-26082
- Clonality： mAb
- Application： ELISA, WB, IF, IHC
- Synonyms： EGFR (L858R)
-
规格：
100ul
-
价格：
¥4800
定制服务咨询
Anti-EGFR (L858R) Mouse Monoclonal Antibody
| Cat. #: TD-26082 | |
| Gene Symbol: EGFR/HER1/ERBB1 | |
| Description: Anti-EGFR(L858R) Mouse Monoclonal Antibody | |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). | |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin. | |
| Applications: ELISA, WB, IF, IHC | |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG1 | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Recognizes EGFR(L858R), but not wild type EGFR from vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. | |
|
Western blot:
Immunofluorescence:
Immunohistochemistry:
|
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L858R) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR L858R) plasmid (right column), then fixed and stained with anti- EGFR(L858R) monoclonal antibody (Cat. # TD-26082).
Immunohistochemical analysis of paraf\fin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (Cat. #TD-26082). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:100) was used as secondary antibody. |