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Anti-EGFR(G719S) Mouse Monoclonal Antibody
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TD-260831
TD-260832
- Catalog： TD-26083
- Clonality： mAb
- Application： ELISA, WB, IHC, IF
- Synonyms： EGFR (G719S)
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规格：
100ul
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价格：
¥4800
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Anti-EGFR(G719S) Mouse Monoclonal Antibody
| Cat. #: TD-26083 |
| Gene Symbol: EGFR |
| Description: Anti-EGFR(G719S) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer. |
| Immunogen: A synthetic peptide from the internal region of human EGFR which includes the mutation of G719S. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:2000 IHC: 1:50-1:1000 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
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Western blot:
Immunofluorescence:
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Western blot analysis of recombinant EGFR(G719S) and wild type EGFR proteins. Purified his-tagged EGFR (G719S) protein (aa 702-987) (Cat. #) and wild type EGFR protein (aa 702-987) (Cat. #) were blotted with anti-EGFR(G719S) mouse antibody (Cat. #TD-26083).
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G719S) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G719S) plasmid (right column), then fixed and stained with anti- EGFR (G719S) monoclonal antibody (Cat. #TD-26083).
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