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Anti-IDH2 (R172H) Mouse Monoclonal Antibody
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TD-260841
TD-260842
- Catalog： TD-26084
- Clonality： mAb
- Application： ELISA, WB, IHC, IF
- Synonyms： IDH2(R172H)
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规格：
100ul
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价格：
¥4800
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Anti-IDH2 (R172H) Mouse Monoclonal Antibody
| Cat. #:: TD-26084 | |
| Gene Symbol: IDH2 | |
| Description: Anti-IDH2(R172H) Mouse Monoclonal Antibody | |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. | |
| Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H. | |
| Applications: ELISA, WB, IHC, IF | |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IF: 1:50-1:100 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. | |
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Western blot:
Immunofluorescence:
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Western blot analysis of recombinant IDH2(R172H) and wild type IDH2 proteins.
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172H) plasmid (right column), then fixed and stained with Anti-IDH2(R172H) monoclonal antibody (Cat. #TD-26084). |