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Anti-EGFR (V769L) Mouse Monoclonal Antibody
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TD-261211
TD-261212
- Catalog： TD-26121
- Clonality： mAb
- Application： ELISA, WB, If, IHC
- Synonyms： EGFR(V769L)
-
规格：
100ul
-
价格：
¥4800
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Anti-EGFR (V769L) Mouse Monoclonal Antibody
| Cat. #: TD-26121 | |
| Gene Symbol: EGFR/HER1/ERBB1 | |
| Description: Anti-EGFR(V769L) Mouse Monoclonal Antibody | |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). | |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of V769L, human origin. | |
| Tested Applications: ELISA, WB, If, IHC | |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Recognizes EGFR(V769L), but not wild type EGFR from vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. | |
|
Western blot:
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Western blot analysis of recombinant EGFR(V769L) and wild type proteins. |
|
Immunofluorescence: |
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(V769L) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(V769L) plasmid (right column), then fixed and stained with anti- EGFR(V769L) monoclonal antibody (Cat. # TD-26121). |