Cat. #: TD-26138 |
Size: 100 µL |
Gene Symbol: PIK3CA |
Description: Anti-PIK3CA(Q546K) Mouse Monoclonal Antibody |
Background: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform. Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Belongs to the PI3/PI4-kinase family. Located to cytosol, lamellipodium and phosphatidylinositol 3-kinase complex. |
Immunogen: A synthetic peptide from the internal region of PIK3CA(Q546K) of human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
Concentration: 0.2 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: recognizes Q546K mutant, but not wild type PIK3CA of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
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Western blot analysis of PIK3CA wild type and Q546K proteins. Equal amount of purified PIK3CA(Q546K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti-PIK3CA(Q546K) mouse monoclonal antibody(Cat. # TD-26138). |