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Anti-IDH2 (R172W) Mouse Monoclonal Antibody
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TD-261641
TD-261642
  • Catalog： TD-26164
  • Clonality： mAb
  • Application： ELISA, WB, IF, IHC
  • Synonyms： IDH2(R172W)
  • 规格：
    100ul
  • 价格：
    ¥4800
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Anti-IDH2 (R172W) Mouse Monoclonal Antibody

Catalog Number:  TD-26164
Gene Symbol:  IDH2(R172W)
Description:   Anti IDH2(R172W) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas.
Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes IDH1(R172W) of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
TD-261641.jpg Western blot analysis of recombinant IDH2(R172W) and wildtype proteins. Purified His-tagged IDH2(R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (Cat. #TD-26164).
Immunofluorescence:    
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R172W) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH2 (WT) plasmid (left column) or pCDNA3-GFP IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (Cat. #TD-26164).