Anti-Human IgE/IGHE Antibody (SAA0342) (TD-HV064023) is amouse monoclonalantibody detectingImmunoglobulin heavy constant epsiloninELISA, FCM. Suitable forHuman.HighlightsFlow Cytometry— Validated for flow cytometry applications.
Species reactivity
Human
Applications
ELISA, FCM
Host species
Mouse
Isotype
IgG1, kappa
Clone ID
SAA0342
Clonality
Monoclonal
Target
Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE
Purification
Protein A/G purified from cell culture supernatant.
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Application
ELISA
Dilution
1:2000-1:20000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Caption
Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # TD-HV064023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # TD-MF690414) and cells analysed on a NovoCyte Flow Cytometer. | SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0342).