TD-HV263016

Brodalumab(TD-HV263016) is a research-grade recombinant antibody targetingCD217. Produced in mammalian cells with native-like glycosylation.HighlightsResearch Grade— For PK/PD studies, assay development, and ADA research.Native Glycosylation— Mammalian expression ensures native-like patterns.

Human

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Human

IgG2-kappa

Monoclonal

IL17R, CDw217, IL-17RA, Interleukin-17 receptor A, IL-17 receptor A, IL17RA, CD217

Please contact the lab for this information.

>95% purity as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Q96F46

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

AMG827, KHK4827, 1174395-19-7

Brodalumab (AMG 827) is a human anti-interleukin-17-receptor IgG2 monoclonal antibody that can be used for the research of moderate-to-severe plaque psoriasis and rheumatoid arthritis.• From Messengers to Receptors in Psoriasis: The Role of IL-17RA in Disease and Treatment., PMID:34201664• Evaluation of interleukin-17 receptor (IL-17RA) gene expression in PBMCs of patients with premature coronary artery disease., PMID:35913009• Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling., PMID:38470486• Mechanistic Insights into the Roles of the IL-17/IL-17R Families in Pancreatic Cancer., PMID:37686343• Role and functional mechanisms of IL‑17/IL‑17R signaling in pancreatic cancer (Review)., PMID:39219271• Loss of Interleukin-17RA Expression is Associated with Tumour Progression in Colorectal Carcinoma., PMID:32462420• Associations of IL-17 and IL-17 receptor polymorphisms with Behçet's disease in Denizli Province of Turkey., PMID:36701075• Increased chemokine receptor IL-17RA expression is associated with poor survival in gastric cancer patients., PMID:26261590• Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop., PMID:32696476• Characteristic Pattern of IL-17RA, IL-17RB, and IL-17RC in Monocytes/Macrophages and Mast Cells From Patients With Bullous Pemphigoid., PMID:31572359

SEC-HPLC detection for Research Grade Brodalumab.　　|　SDS-PAGE for Research Grade Brodalumab.　　|　Flow-cytometry using anti-human CD217 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD217 monoclonal antibody (Catalog HV263016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only. Not suitable for clinical or therapeutic use.