TD-HF943016

Canakinumab(TD-HF943016) is a research-grade recombinant antibody targetingInterleukin-1 beta. Produced in mammalian cells with native-like glycosylation.HighlightsResearch Grade— For PK/PD studies, assay development, and ADA research.Native Glycosylation— Mammalian expression ensures native-like patterns.

Human

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Human

IgG1-kappa

Monoclonal

Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta

Please contact the lab for this information.

>95% purity as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P01584

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

ACZ885, 914613-48-2

Rilonacept (Arcalyst), a dimeric fusion protein, is a interleukin 1 inhibitor. Rilonacept consists of the ligand-binding domains of the extracellular portions of the IL-1R components linked to the Fc portion of human IgG1. Rilonacept can be used for the research of cryopyrin-associated periodic syndromes.• Understanding the mechanism of IL-1β secretion., PMID:22019906• NLRP3 inflammasome mediates M1 macrophage polarization and IL-1β production in inflammatory root resorption., PMID:31976565• The role of IL-1β and TNF-α in intervertebral disc degeneration., PMID:32853910• Human Monocytes Engage an Alternative Inflammasome Pathway., PMID:27037191• D-mannose suppresses macrophage IL-1β production., PMID:33311467• BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity., PMID:29324233• Association of interleukin-6 and interleukin-1-β levels in patients with toxoplasmosis., PMID:35782407• Interleukin-1 beta is significantly upregulated in the decidua of spontaneous and recurrent miscarriage placentas., PMID:33545613• The effect of interleukin 1 beta blockade on the tumor microenvironment., PMID:33493144• Single-cell profiling identifies IL1Bhimacrophages associated with inflammation in PD-1 inhibitor-induced inflammatory arthritis., PMID:38453911

Detects Human IL1B/IL1F2 in indirect ELISAs.　　|　SDS-PAGE for Research Grade Canakinumab　　|　Flow-cytometry using PE anti-human IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL1B monoclonal antibody (Catalog HF943016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. ### Flow-cytometry using anti-human IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1B monoclonal antibody (Catalog HF943016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only. Not suitable for clinical or therapeutic use.