TD-HF802016

Research Grade Bermekimab(TD-HF802016) is ahumanized monoclonalantibody detectingIL1F1inELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.

Human

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Humanized

IgG1-kappa

Human

Monoclonal

IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

P01583

Liquid

0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

MABp1, Xilonix, CAS: 1401965-15-8, CA-18C3, CV-18C3, RA-18C3, T2-18C3

SEC-HPLC detection for Research Grade Bermekimab.　　|　Detects IL1A/IL1F1 in indirect ELISAs.　　|　SDS-PAGE for Research Grade Bermekimab.　　|　Flow-cytometry using PE anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. ### Flow-cytometry using FITC anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. ### Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

For research use only. Not suitable for clinical or therapeutic use.