3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Applications
Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Species
HRV-14
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Form
Liquid
Storage buffer
50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, Please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Caption
SDS-PAGE for 3C Protease | M: Marker1: Control2: Fusion protein digest with 3C proteaseCase studyFormulaconcentration1 mg/mlc mg/mlvolume100 µlV mlsubstrate100 µgcV mg10X Buffer11 µlV/9 ml3C Protease1 µlcV/100 mlMix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.
抗体
试剂盒
多肽
蛋白
组织芯片
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