Endo S2/Endo-beta-N-acetylglucosaminidase is cloned from Streptococcus pyogenes and expressed in E.coli.
Applications
Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
Species
Streptococcus pyogenes
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from PBS pH7.4, 1 mM EDTA, 4%trehalose, 1% mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
SDS-PAGE for Endo S2/Endo-beta-N-acetylglucosaminidase | Human IgG1 was incubated with (+) or without (-) Endo S2 and analyzed by reducing SDS–PAGE. A reduced molecular weight of the heavy chain was observed in the Endo S2–treated group.